Workflow for isolating viable human central nervous system immune cells in STAR Protocols

31 march 2026

This method provides a workflow for isolating primary microglia, B cells, and T cells from human central nervous system (CNS) samples. The protocol outlines standardized procedures for extracting immune cells from multiple CNS compartments, including brain samples, choroid plexus, leptomeninges, dura mater, cerebrospinal fluid (CSF). Relevant downstream applications include cell sorting, RNA sequencing, and functional assays using primary cultures.

Interested in applying primary immune cells in your research? Contact the NBB Service team.

Read the full publicaton: STAR Protocols