NBB-Psy, the Netherlands Brain Bank for Psychiatry
NBB-Psy is a program of the Netherlands Brain Bank. Its aim is to establish a resource of brain tissue of 7 major psychiatric disorders:
- Major depression
- Bipolar disorder
- Obsessive-compulsive disorder
- Post-traumatic stress disorder
- Autism spectrum disorder
- Attention-deficit hyperactivity disorder
As the personal, social and economic burden of psychiatric disorders is high, there is an urgent need for better treatment strategies, which requires the understanding of underlying etiology and pathophysiology of psychiatric disorders. The use of human brain tissue provides the most direct strategy to develop and test hypotheses about the molecular and cellular basis of psychiatric disorders. The current availability of human brain tissue from patients with psychiatric disorders is by far not sufficient. Therefore, it is our mission to develop a qualitatively unique tissue program to increase the number of post-mortem brains of well-characterized psychiatric and control donors: NBB-Psy (NHB-Psy in Dutch). This resource is now available to the national and international research community via the application procedures of the NBB.
To make NBB-Psy possible, in 2012, the NBB received a grant of 3.45 million Euros from the Netherlands Organisation for Scientific Research (NWO), in collaboration with five Dutch university medical centers: UMC Utrecht, VUmc & AMC Amsterdam, Radboudumc Nijmegen and Erasmus MC Rotterdam. With this grant the NBB and the five university medical centers have been working on a national infrastructure with the goal of collecting brain tissue for research purposes, from well-diagnosed persons with psychiatric diagnoses. Direct access to brain tissue can facilitate breakthroughs in researching the origins of psychiatric diseases. This is important, because psychiatric diseases elicit many questions that cannot be sufficiently answered at this time.
Additional data and samples for NBB-Psy cases
Apart from the standard information that is provided together with the samples (extensive summary of medical records and neuropathological report), for NBB-Psy additional information and material will be available:
- Antemortem data within NBB-Psy
All potential donors have to fill in a medical questionnaire when they register. In addition, we will use web-based questionnaires to generate five-yearly updates on relevant clinical parameters.
- Postmortem enrichments within NBB-Psy
We will use novel methods to enrich the brain material by:
- Isolation of primary microglia
Pure microglia are isolated from white matter and occipital cortex. Isolated microglia are phenotyped using flow cytometric analysis and stored as lysates for RNA analysis or cryogenically frozen. Both are currently available to researchers via e-NBB.
- Generation of microglial cell lines from pure microglial cells for high throughput cell biological analyses.
- Biobank for molecular analyses of the diverse disorders
Due to the increased demand for tissue in molecular analyes (RNA, DNA, protein), and the scarcity of certain brain regions, NBB-Psy has reserved certain frozen tissue blocks specifically for these research projects. Upon request, NBB-Psy will provide researchers with a specific amount of tissue suitable to the project. This ensures that multiple research projects can include the same donors and brain regions, and ensures the continued availability of these regions for future studies.
- Human induced pluripotent stem cells (iPSC’s)
- Isolation of primary microglia
Fibroblasts have been isolated (from skin fibroblasts) of major depression, schizophrenia, bipolar disorder, autism spectrum disorder, post-traumatic stress disorder and control donors. These fibroblast cultures have been reprogrammed into pluripotent stem cell lines and will be made available to researchers as cryogenically frozen samples. Through published protocols, researchers can obtain neural stem cells, and subsequently acquire donor-specific glial cells and neurons. A list of iPSC lines and donor characteristics can be found in the NBB iPSC document. Note we also have brain tissue available from these donors for validation (refer to our tissue availability page and eNBB database). Please email your iPSC/tissue application to the NBB (eNBB@nin.knaw.nl) or contact us if you have any questions
The iPSCs are derived at the Erasmus Medical Centre IPS core facility using a fast epigenetic silencing lentiviral vector with a multicistronic reprogramming vector carrying Oct4, Klf4, Sox2 and c-Myc (Warlich et.al., 2011). It also carries a red fluorescent marker to monitor silencing of reprogramming factors and the selection of thoroughly reprogrammed iPS clones. These lines are derived and cryopreserved on irradiated mouse embryonic fibroblasts in Human ES medium. From each line several validated clones are banked. Each clone has an associated document providing information about culture, cryopreservation and quality controls. Clones can be provided in common feeder free media at additional cost.
 Warlich E, Kuehle J, Cantz T, Brugman MH, Maetzig T, Galla M, Filipczyk AA, Halle S, Klump H, Schöler HR, Baum C, Schroeder T, Schambach A. Lentiviral vector design and imaging approaches to visualize the early stages of cellular reprogramming. Mol Ther. 2011 Apr;19(4):782-9. doi: 10.1038/mt.2010.314. Epub 2011 Feb 1. PMID: 21285961; PMCID: PMC3070104.