The NBB can provide samples of more than 4000 autopsies of donors with a variety of neurological and psychiatric disorders or non-demented controls. The NBB has an open access policy and researchers affiliated to universities, other non-profit organizations, and pharmaceutical companies from all over the world can apply for samples.
Tissue is dissected from different anatomical regions and stored in various ways. The NBB also provides CSF, plasma and sometimes the spinal cord and dorsal root ganglia. On request, the specimens can be obtained fresh and placed in special media or fixatives according to the researcher’s protocol. DNA samples are available of part of the collection. Requests for specific brain areas are taken into account when performing autopsies.
The tissue is accompanied by anonymized donor information, including the neuropathological report and an extensive summary of the donor’s medical history. If requested, the tissue can be matched for ante-mortem parameters (e.g. age, sex, time and season of death, agonal state, pH, APO-E genotype) and post-mortem parameters (post-mortem delay, freezing or fixation procedure, storage time).
Online database access: e-NBB
The e-NBB provides online access to the NBB database. This enables researchers to find detailed information on current tissue availability. Furthermore, it is possible to make your own tissue selection, matched for ante-mortem and / or post-mortem parameters if necessary. To request tissue, this selection can be sent to the NBB together with the application form.
Tissue availability in general
Tissue is available in three main storage types: frozen, formalin-fixed and paraffin embedded (FFPE), and culture medium (fresh samples). Upon request, paraffin sections (8 micron) or formalin-fixed (not paraffin embedded) samples can also be provided. The brain areas of which tissue is generally available are listed below.
- Cortex (Frontal, Temporal, Parietal, Occipital)
- Locus coeruleus
- Substantia nigra
- Medulla oblongata
- Spinal cord
- Dorsal root ganglia
- Olfactory bulb
- Normal appearing white matter
- MS lesions (active, inactive)
- MS lesions dissected on MRI guidance
- Cerebrospinal fluid
- Microglial cells
- Human induced pluripotent stem cells (iPSC’s) from well-characterised psychiatric and control donors registered through the NBB-Psy donor program.
- Eyes (more information is available on the sub-page about eye donation)
Although the e-NBB provides the most recent and accurate information on tissue availability, this table provides a general overview of the availability levels of the different brain areas and types of storage, specified by diagnosis. Please note that for MS different brain areas are specified, because MS brains are dissected using a specific protocol.
NBB in publications
When using NBB tissue, please mention the Netherlands Brain Bank as the source in the Material & Methods section or equivalent. In addition, the NBB asks researchers to consider corporate authorship of the NBB for publications that required significant intellectual input from NBB staff. The NBB authorship guidelines contain more detailed information. Dr. Sophie Wehrens, Scientific Coordinator of the NBB, will review manuscripts where the NBB is listed as an author. The NBB can be added to the author list as ‘Netherlands Brain Bank’, or in case of analysis of donors with a psychiatric diagnosis, "Netherlands Brain Bank for Psychiatry". A corporate author is preceded with a ; (not a , ). The affiliation of the NBB is: Netherlands Institute for Neuroscience, Meibergdreef 47, 1105 BA, Amsterdam. For questions on publications or to get your manuscript reviewed, please email firstname.lastname@example.org or eNBB@nin.knaw.nl.